RC-Hydrolase is the first actiome (i.e., active site database) for three-dimensional structural data of catalytic/active sites of single and multi-chain enzyme molecules listed in the Protein Data Bank (PDB), created by the Chowdhury Lab at Iowa State University. This database enables comparison and visualizing structurally similar, yet functionally different active pockets, from desired host organisms thereby offering versatile starting scaffolds to design multi-functional enzymes for industrial biotechnology. RC-Hydrolase scaffolds can be fine-tuned using enzyme engineering through integer optimization-based (IPRO) or machine learning-based (ProteinMPNN) for high catalytic efficiency.
CADseek, the 3D shape search engine by the iSEEK Corporation, indexed RC-Hydrolase and is used here for performing real-time searches of the 3D structural data of catalytic/active sites of single and multi-chain enzyme molecules listed in the Protein Data Bank (PDB) as well as for generating Big Data Shape Analytics reports for the dataset.
Contact:
Ratul Chowdhury (ratul@iastate.edu)
Please cite RC-Hydrolase